专利内容由知识产权出版社提供
专利名称：METHODS FOR CULTURING MAMMALIAN
TASTE CELLS
发明人：RAWSON, Nancy Ellen,OZDENER, Mehmet
Hakan
申请号：EP05810194.0申请日：20051013公开号：EP1833961A2公开日：20070919
摘要：The invention provides methods of culturing mammalian taste cells, includingtaste receptor cells. Cells are maintained for a duration of up to three months andlonger while maintaining molecular and functional characteristics of mature taste cells.The cells are cultured on coated cell culture vessels and, from first replacement ofmedium onwards, the medium is replaced in intervals of at least 5 days. The inventionfurther provides isolation and culturing methods of taste cells wherein the time that thecells are exposed to isolation solution and proteolytic enzymes is minimized and the cellsare cultured in coated culture vessels with the medium replaced in intervals of at least 5days from first replacement onwards. The invention further provides cultured taste cells,transfection and assay methods, and taste cell assay buffers with an osmolarity of about300-320 and pH of about 7.0-7.3.
申请人：MONELL CHEMICAL SENSES CENTER
地址：3500 Market Street Philadelphia, PA 19104-3308 US
国籍：US
代理机构：McStea, John Anthony
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