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专利名称：EFFICIENT NUCLEIC ACID ENCAPSULATION
INTO MEDIUM SIZED LIPOSOMES
发明人：TONG, SHANGGUAN,LI, XINGONG,MEERS,
PAUL, R.,PERKINS, WALTER, R.,POLOZOVA,ALLA
申请号：EP03703717申请日：20030108公开号：EP1478341A4公开日：20090520
摘要：A method for preparing liposomes containing at least one nucleic acidencapsulated therein comprising the following steps: (A) mixing a gel or a liquidcontaining gel particles with aqueous medium Z1 to directly form the liposomes
containing the at least one nucleic acid encapsulated therein; (B) (i) mixing a gel or a liquidcontaining gel particles with aqueous medium Z1 to form a curd or curdy substance; and(ii) mixing the curd or curdy substance with aqueous medium Z2 to directly form theliposomes containing the at least one nucleic acid encapsulated therein; or (C) (i) cooling agel or a liquid containing gel particles to form a waxy substance; and (ii) mixing the waxysubstance with aqueous medium Z1 to directly form the liposomes containing the atleast one nucleic acid encapsulated therein; wherein said gel or liquid containing gelparticles comprises at least one liposome-forming lipid, at least one fusogenic lipid, awater-miscible organic solvent and the at least one nucleic acid; wherein an amount of theat least one fusogenic lipid is at least 20% by weight of a lipid content of the gel or theliquid containing gel particles; and wherein the aqueous media Z1 and Z2 are the same or
different.
申请人：TRANSAVE, INC.
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