BV421

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Version: 1 Revision Date: 2012-11-30

Product Data SheetBrilliant Violet 421™ anti-mouse/human CD11b

Catalog # / Size:101235 / 125 µl

101251 / 50 µg101236 / 500 µl

Clone:M1/70Isotype:Rat IgG2b, κ

Immunogen:C57BL/10 splenocytes

Reactivity:Mouse, Human, Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus,

Rhesus, Rabbit (Lapine)Preparation:The antibody was purified by affinity chromatography and conjugated with

Brilliant Violet 421™ under optimal conditions. The solution is free ofunconjugated Brilliant Violet 421™ and unconjugated antibody.Formulation:Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and

BSA (origin USA).Concentration:µg sizes: 0.2 mg/ml

µl sizes: lot-specific (please contact technical support for concentrationand total µg amount)

Storage:The antibody solution should be stored undiluted between 2°C and 8°C,

and protected from prolonged exposure to light. Do not freeze.

BL/6 mouse lymph nodes, fixed O/Nin PLP, blocked with 10% rat serum,stained with CD11b-BV421™ (red),B220-Alexa Fluor® 7 (blue),

CD14-FITC (green) in 1% BSA and0.1% Tween-20 in PBS. Images wereacquired with an automated widefieldmicroscope (Nikon Eclipse Ti) and aCCD camera (QImaging Retiga

2000R). Emitted light was collectedthrough 440/40, 525/50, and 700/75nm bandpass filters. Images providedby Ann Haberman and ChristinePodolski, Yale University.

Applications:

Applications:FC - Quality tested

IHC - Validated

Recommended Usage:Each lot of this antibody is quality control tested by immunofluorescent

staining with flow cytometric analysis. For flow cytometric staining usingthe µg size, the suggested use of this reagent is ≤0.25 µg per million cellsin 100 µl volume. For flow cytometric staining using the µl sizes, the

suggested use of this reagent is ≤5 µl per million cells or 5 µl per 100 µl ofwhole blood. It is recommended that the reagent be titrated for optimalperformance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standardbandpass filter 450/50 nm is recommended for detection. Brilliant Violet421™ is a trademark of Sirigen Group Ltd.

Application Notes:Additional reported applications (for relevant formats of this clone)

include: immunoprecipitation1,4, in vitro blocking3,9,12, depletion2,8,immunofluorescence microscopy6,7,10, and immunohistochemistry ofacetone-fixed frozen sections5,11-13 and paraffin sections28. The LEAF™purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) isrecommended for functional assays (Cat. No. 101231). For in vivo studiesor highly sensitive assays, we recommend Ultra-LEAF™ purified antibody(Cat. No. 101248) with a lower endotoxin limit than standard LEAF™purified antibodies (Endotoxin <0.01 EU/µg).Application References:1. Springer T, et al. 1978. Eur. J. Immunol. 8:539. (IP)

2. Ault K and Springer T. 1981. J. Immunol. 126:359. (Deplete)3. Springer TA, et al. 1982. Immunol. Rev. 68:171. (Block)

4. Ho MK and Springer TA. 1983. J. Biol. Chem. 258:2766. (IP)5. Flotte TJ, et al. 1983. Am. J. Pathol. 111:112. (IHC)6. Noel GJ, et al. 1990. J. Clin. Invest. 85:208. (IF)

7. Allen LA and Aderem A. 1996. J. Exp. Med. 184:627 (IF)8. D'Amico A and Wu L. 2003. J. Exp. Med. 198:293. (Deplete)9. Brickson SJ, et al. 2003. Appl Physiol. 95:969. (Block)

10. Clatworthy MR and Smith KG. 2004. J. Exp. Med. 199:717. (IF)11. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)12. Zhang Y, et al. 2002. J. Immunol. 168:3088. (IHC)

13. Iwasaki A and Kelsall BL. 2001. J. Immunol. 166:4884 (IHC, FC)14. Tailleux L. 2003. J. Exp. Med. 197:121. (Block, FC)15. Olver S, et al. 2006. Cancer Research 66:571. (FC)

C57BL/6 mouse bone marrow cellswere stained with CD11b (cloneM1/70) Brilliant Violet 421™ (filledhistogram) or rat IgG2b, κ BrilliantViolet 421™ isotype control (openhistogram). Data shown was gated onmyeloid cell population.

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible

for patent infringement or other violations that may occur with the use of our products.

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products maynot be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to thirdparties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, theseproducts are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

BioLegend Inc., 9727 Pacific Heights Blvd, San Diego, CA 92121 www.biolegend.comToll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

16. Tan SL, et al. 2006. J. Immunol. 176:2872. (FC) PubMed 17. Ponomarev ED, et al. 2006. J. Immunol. 176:1402. (FC)18. Dzhagalov I, et al. 2007. Blood 109:1620. (FC)19. Fazilleau N, et al. 2007. Nature Immunol. 8:753.

20. Rasmussen JW, et al. 2006. Infect. Immun.74:6590. PubMed21. Napimoga MH, et al. 2008. J. Immunol. 180:609. PubMed

22. Elqaraz-Carmon V, et al. 2008. J. Lipid. Res. 49:14. PubMed23. Kim DD, et al. 2008. Blood 112:1109. PubMed24. Guo Y, et al. 2008. Blood 112:480. PubMed

25. Norian LA, et al. 2009. Cancer Res. 69:3086. (FC) PubMed26. Baumgartner CK, et al. 2010. J. Immunol. 184:573. PubMed27. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed

28. Whiteland J, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)29. Weber GF, et al. 2014. J Exp Med. 211:1243. PubMed30. Price PJ, et al. 2015. J Immunol. 194:11. PubMed31. Doni A, et al. 2015. J Exp Med. 212:905. PubMed

Description:CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α

subunit, Mol, CR3, and Ly-40. CD11b is a member of the integrin family,primarily expressed on granulocytes, monocytes/macrophages, dendriticcells, NK cells, and subsets of T and B cells. CD11b non-covalentlyassociates with CD18 (β2 integrin) to form Mac-1. Mac-1 plays an

important role in cell-cell interaction by binding its ligands ICAM-1 (CD54),ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen.Other Names:αM integrin, Mac-1, Mo1, CR3, Ly-40, C3biR, ITGAM

Antigen References:1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic

Press.

2. Springer TA. 1994. Cell 76:301.

3. Coxon A, et al. 1996. Immunity 5:653.

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BV421