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专利名称：Methods and compositions of amplifying
RNA
发明人：Stephen D. Ginsberg,Shaoli Che申请号：US10075335申请日：20020214
公开号：US20030186237A1公开日：20031002
摘要：The present invention pertains to a method that will increase the efficiency ofsecond strand cDNA synthesis through a mechanism of “terminal continuation” beforefurther RNA amplification by RNA transcription using, for example, a bacteriophagepromoter. In a specific embodiment, a transcription promoter is attached to the 5′ regionof cDNA through the same mechanism of “terminal continuation”. Genetic signals aresubsequently amplified in a linear manner through RNA transcription. In specificembodiments, the orientation of the transcribed RNA is either sense or antisense,depending on the desired downstream application. In other embodiments, the presentinvention pertains to methods for extraction and amplification of RNA, particularly mRNA,from histologically stained tissues and cells.
申请人：BAYLOR COLLEGE OF MEDICINE
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