三激光九色超高速流式分选仪
参数要求：
1， 用于各类细胞的分析和分选,特别要求用于各类肿瘤干细胞SP分选，SP含量在万分之一至万分之五的细胞达到99%纯度的分选；
2， 激光器1: 488nm Solid State 200mW；
3， 激光器2: 355nm(UV) Solid State 100mW；
4， 激光器3: 0nm Diode 100mW；
5， 选；
可选配高功率水冷激光器,激发458nm谱线达300mW，可用于染色体分
6， 可选配激光共线器，激光共线器可安装的激光器波长：405nm, 488nm,
532nm, 561nm, 592nm,0nm；
7， 开放的光学工作台，可进行光学升级，可装备最多激光数为9个；
8， *分析速度:>100,000 events/sec（在任何数量荧光和补偿下）；
9， *分选速度: >70,000 events/sec（在任何数量荧光和补偿下）；
10， *液滴振荡频率: 200KHz；
11， 信号处理器（ADC）数量：2个；
12， 电子系统信号处理频率:100MHz；
13， 荧光线形范围：5个对数阈；
14， 光学检测参数: 2个散射光，可增加至22个光学检测参数；
15， *分选纯度: >99%；
16， 分选通道: 二、四路分选；
17， 细胞模式；
同时分选的四个通道可以选择不同的分选模式：富集模式，纯化模式或单
18， 同一群细胞可以同时采用两种模式分别收集到两个管中，其中一支管得到
高纯度的细胞而另一管得到剩余的全部细胞；
19， 标配单细胞分选: 可做单细胞分选, 可将细胞分选到6，24，96，384，
1536孔板内，亦可将细胞分选到载玻片上形成1536个细胞的细胞芯片，及客户自定义矩阵；
20， *信号脉冲分辨率: 32比特，即为42.9亿道；
21， 可选喷嘴大小: 8种喷嘴可选，50 um、7O um、80 um、90 um、1OO um、
120 um、150 um、200 um，可满足小至0.2um,大于100um的细胞或颗粒的检测；
22， 最小检测颗粒大小: <0.2 um；
23， 系统压力: 4-100psi；
24， 系统操作界面: 触摸屏图形控制器；
25， 进样装置: 全自动上样平台，上样管可以为：0.5 mL，1.5 mL，5 mL tube，
14 mL round，15 mL，50 mL；
26， 具有自动气泡检测功能，具有自动去泡、搅动、冲洗功能；
27， 和操作控制；
数据分析系统: 双电脑服务器配置，分别由负责电子信号处理和数据处理
28， 数据分析软件：安装和使用没有版权，软件可以任意安装在其他计算
机上，方便用户在任意个人电脑上分析数据；
29， *单次获取和储存的细胞信息量:10亿个；
30， *无需微球的液滴延迟时间（Drop Delay）计算自动化；
31， 在温度变化为±3℃,压力变化在±2PSI情况下保持原来的液流自动监测和自动稳定,完全无需人为操作液滴延迟的自动控制；
32， 网络连接的自动实验设计功能，使荧光搭配更科学，更简单，多色分选更可靠；
33， 要求国内有多家良好应用参照。
Ultra high speed flow sorter bidding parameters
Parameter requirements:
1, used for various types of cell analysis and sorting, special requirements for all kinds of tumor stem cell sorting SP, SP content of 99% purity in one over ten thousand to five over ten thousand of the cells sorting;
1:2, laser of 488 nm Solid State 200 mw;
2:3, laser of 355 nm (UV) Solid State 100 mw;
4, laser 3:635 nm Diode 25 mw;
5, optional high-power water-cooled laser, stimulate the 458 nm line of 300 mw, can be used for chromosome separation.
6, optional laser collinear, laser wavelength of laser collinear device can be installed: 405 nm, 488 nm, 532 nm, 561 nm, 592 nm, 0 nm;
7, open optical bench, can upgrade the optical, can be equipped with a laser number for up to nine;
8, analysis speed: > 100000 events/SEC () in any number of fluorescence and compensation;
9, sorting speed: > 70000 events/SEC () in any number of fluorescence and compensation;
10, droplet oscillation frequency, 200 KHZ;
11, number of signal processor (ADC) : 2;
12, electronic signal processing system frequency: 100 MHZ;
13, fluorescent linear range: 5 logarithmic threshold;
14, optical detection parameters: two scattering light, can be increased to 22 optical detection parameters;
15, sorting, purity > 99%;
16, the separation channel: 2, 4 road separation;
17, at the same time, separation of the four channels can choose different separation modes: enrichment patterns, purification mode or single cell;
18, the same group of cells can adopt two modes separately collected at the same time two tubes, one of the cells of the tube with high purity and another tube to get all the rest of the cells;
19, standard single cell sorting: can do single cell separation, cell separation can be to 6,24,96,384,1536 orifice, or cell sorting to 1536 cells cell chip is formed on the glass slide, and customer custom matrix;
20, signal pulse resolution: 32 bits, is 4.29 billion;
21, optional nozzle size: optional eight kinds of nozzle, 50 um, um, 7 o 1 oo um um, um, 90, 80 120 150 150 um, um, um, can meet the small to 0.2 um, more than 100 um cells or particles detection;
22, the minimum detectable particle size: < 0.2 um.
23, the system pressure: 4-100 psi.
24, system operation interface: touch screen graphics controller;
25, sampling device: fully automatic sample on the platform, can be as follows: on the 0.5 mL and 1.5 mL and 5 mL tube, 14 mL round, 15 mL and 50 mL;
26, with functions of automatic air bubble detection, with functions of automatic to bubble, agitation, flushing;
27, the data analysis system: double computer server configuration, respectively, by the responsible for digital signal processing and data processing and operation control;
Installation and use of 28, data analysis software: no copyright restrictions, the software can be installed on other computers, any convenient user on any personal computer analysis of data;
29, single cell information acquisition and storage: 1 billion;
30, no Delay time of droplets (Drop Delay) calculation automation;
31, the temperature changes of plus or minus 3 ℃, pressure change under the condition of plus or minus 2 psi to keep the original flow automatic monitoring and automatic stabilization, fully automatic control without artificial operation droplet delay;
32, the network connection of automatic design of experiment, the fluorescent collocation is more scientific, more simple, more color separation more reliable;
33 to have several good application reference.