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专利名称：Method for purifying interferon and
composition of matter produced thereby
发明人：Arakawa, Tsutomu申请号：EP86302960申请日：19860418公开号：EP0199568A3公开日：19880921
摘要：A composition of matter consisting of recombinant human immune interferonhaving a near-UV circular dichroic spectrum in aqueous solution at neutral pH withpositive bands at about 259 nm, 266 nm, 280 nm, and 287 nm, and with shoulders at about270 nm and 292 nm. Also disclosed is a method for purifying human immune interferon inwhich proper refolding of the interferon is accomplished by unfolding in a denaturant,such as urea, dilution in ammonium acetate to approximately 0.18 mg/ml of interferon (orless), and dialysis of the solution. The properly folded, purified product which results hasa four- to eight-fold greater activity than the aggregate which otherwise results.
申请人：Amgen
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